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Breaking Down Glycosides in the Gut and in Nature

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Researchers from University of Tsukuba articulate the mechanism involved in the breakdown of sugar-bearing molecules known as C-glycosides


Tsukuba, Japan—Rarely is a tool more useful when it's broken, but that's just the case with C-glycoside, a molecule found in many plants, foods, and medicines. To be used by the body, C-glycosides must be broken down. Researchers in Japan have uncovered new insights into how this process occurs.


In a study published in Nature Communications, researchers from University of Tsukuba have shed new light on the mechanism involved in the metabolism of C-glycosides, which contain a sugar group that is attached via a carbon-carbon (C-C) bond.


Humans regularly ingest C-glycosides found in fruits and vegetables. Breakdown of C-glycosides via the cleavage of the C-C bond occurs in the large intestine and is necessary for the body to utilize these molecules. However, the catalytic mechanisms involved in this process are not fully understood. Researchers at University of Tsukuba investigated the mechanism and components involved in C-glycoside metabolism in the human body and in nature.


"Using assimilation screening and genome mining, we were able to identify multiple C-glycoside deglycosylation enzymes present in intestinal bacteria and soil bacteria," says senior author Michihiko Kobayashi.


The research team used biochemical techniques to identify the role and specificity of C-glycoside deglycosylation enzymes (CGDs) in the metabolism of C-glycosides. Structural analysis revealed the unique structures of the CGDs from the gut and soil bacteria and illustrated the relationship between the enzyme structure and function.


"We found that the CGDs from both intestinal and soil bacteria functioned as catalysts for selective C-C bond cleavage reactions," explains author Takuto Kumano. "Per our analyses, we propose a C-C bond cleavage mechanism involving acid/base catalysis in the breakdown of C-glycosides."


The researchers observed that the structure of CGDs in intestinal bacteria differed from that of CGDs in soil bacteria. However, the nature of the reaction for C-glycoside metabolism appeared to be common in soil and intestinal microorganisms.


Glycosides occur throughout nature and are present in certain medicines that treat, for example, heart failure. Further understanding of the enzymes involved in C-glycoside metabolism will provide more insight into how the body breaks down these molecules for use and how this process may be used for the activation of drugs containing C-glycosides.


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This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (JSPS KAKENHI Grant Numbers JP16H06443, JP19K15703, JP19K05784, JP19H05687, JP20H00490, JP20K22700, and JP21K18246), the New Energy and Industrial Technology Development Organization (NEDO, Grant Number JPNP20011), the PRESTO program of the Japan Science and Technology Agency, and the Fuji Foundation for Protein Research. This work was also supported in part by Platform Project for Supporting Drug Discovery and Life Science Research (Basis of Supporting Innovative Drug Discovery and Life Science Research) from AMED (JP21am0101071 (support number 1553) to T.S., KEK).

Original Paper

The article, "C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes," was published in Nature Communications at DOI: 10.1038/s41467-021-26585-1


Correspondence

Professor KOBAYASHI Michihiko
Faculty of Life and Environmental Sciences, University of Tsukuba


Related Link

Faculty of Life and Environmental Sciences